You have to upload 4 things to access HECNet:

MAKE SURE THE SAME SEQUENCE IS USED FOR ALL THE FEATURES.

1) Sequence should be in Fasta format.
   When writing the protein sequence into the text area, make sure to consult the 'Sample Sequence File'.
   The sequence should not be less than 50 or greater than or equal to 1000 amino acids long.
   The sequence should not contain amino acids other than the 20 naturally occuring amino acids.    

2) Generate the PSSM of the sequence using https://www.ebi.ac.uk/Tools/sss/psiblast/
   Set the database as UniProtKB/Swiss-Prot (The manually annotated section of UniProtKB)
   Paste the protein sequence in Fasta format.
   Click Submit.
   It will take a little while to run the first iteration.
   After it completes, click 'Run next iteration'.
   Again, it will take some time.
   After completion of second iteration, click 'Run next iteration again'.
   After the third and final iteration completes, click Download PSSM file and upload the downloaded file in the 'Upload PSSM file section'.
   Make sure the file's extension is .pssm.txt

3) To get the functional domain, open the link: https://www.ebi.ac.uk/Tools/hmmer/search/hmmscan
   Enter the sequence in Fasta format.
   Select Pfam as the HMM Database.
   Click Submit.
   After the request is processed, click Download
   Then click the JSON option and the file will be downloaded.
   Upload the file in the Upload Functional Domain file option.
   Make sure the file's extension is .txt

4) To generate the Disordered Regions, Secondary Structure and Solvent Accessibility information, open the link:         
   http://raptorx.uchicago.edu/StructurePropertyPred/predict/
   Enter the sequence in Fasta format.
   Click submit.
   Wait for the server to give the outcome.
   Download the resulting file (in zip format).
   Uncompress the zip file.
   Upload the '.all' file in the 'Upload Deepcnf' section.
   Make sure the extension remains as .all

The prediction can take a few minutes. Including the multi-functional functionality will further increase the time taken.

Please contact us at hammad.naveed@nu.edu.pk in case of any problem.